Tip60 inhibitors

ABSTRACT

This invention is in the fields of immunology and autoimmunity. More particularly it concerns pharmaceutical compositions comprising compounds which are useful agents for inhibiting the functions of TIP60 and the use of such compounds in the treatment of an individual suffering, for example, from ulcerative colitis and other irritable bowel diseases.

CROSS REFERENCE TO RELATED APPLICATIONS

This patent application claims the priority benefit of U.S. ProvisionalApplication Ser. Nos. 61/419,465 and 61/419,473, both filed Dec. 3,2010, each of which is incorporated by reference herein in its entirety.

TECHNICAL FIELD

This invention is in the fields of immunology and autoimmunity. Moreparticularly it concerns pharmaceutical compositions comprisingcompounds which are useful agents for inhibiting the functions of TIP60and in the treatment of an individual suffering, for example, fromulcerative colitis and other irritable bowel diseases.

BACKGROUND

The incidence of inflammatory bowel disease (IBD), including Crohn'sdisease and ulcerative colitis, is currently between 75-150 cases per100,000 individuals in the US and increasing. The development of IBD isinfluenced by an individual's genetic background, immune responses andenvironment (including gut microbiota and toxin exposure). Clinical andexperimental data indicate that the development of IBD is mediatedprimarily by CD4+ T cells. Current best practice therapy for IBDinvolves cytokine targeting using biologic therapies up to and includingparental administration of anti-TNF-α biologics. However, suchtreatments protocols are expensive to produce and deliver, and are proneto induce blocking antibodies that can limit their long-term safety andefficacy.

Clinicians believe that, despite the availability of anti-TNF-αbiologics for the treatment of moderate to severe Crohn's disease andulcerative colitis, there is a major unmet need for drugs that induceand maintain remission without immune suppression and the need forcorticosteroids.

Given continuous exposure of the gut to microbial and other antigens,the regulation of host inflammatory responses by thymic-derived FOXP3+ Tregulatory (Treg) cells is crucial to the maintenance of health. Bothhumans and mice with defects in FOXP3 develop severe autoimmunity,including colitis, and adoptive transfer of Tregs can reverseestablished colitis in murine models, though this is not practical forlong-term clinical therapy. By contrast, identifying ways topharmacologically promote Treg suppressive functions has considerablepotential for therapeutic application in patients with IBD.

Previous research has shown that FOXP3 acetylation and Tregs functionsare controlled by the interactions of histone/protein acetyltransferases(HATs) and histone/protein deacetylases (HDACs). See, for example, Li etal., “FOXP interactions with histone acetyltransferase and class IIhistone deacetylases are required for suppression,” Proc. Natl. acad.Sci. USA, 2007 Mar. 13: 104 (11): 4571-6, which is incorporated byreference herein. Specifically, the HAT enzyme, TIP60, recruits p300 toa FOXP3 complex and p300-mediated acetylation of FOXP3 is required foroptimal Treg functions, whereas autoacetylation of TIP60 promotesdisassembly of this activating complex (see, e.g., FIG. 6).

Increased FOXP3 acetylation can be achieved by use of histone/proteindeacetylase (HDAC) inhibitors, as would be relevant to autoimmunity andtransplant rejection, whereas decreased FOXP3 acetylation can beachieved using histone/protein acetyltransferase (HAT) inhibitors, asmight be useful in various malignancies wherein the Treg population maylimit host anti-tumor responses. TIP60 inhibitors play a special role inpromoting FOXP3 acetylation by prolonging the recruitment of p300 to theFOXP3 signaling complex.

Additional information on this subject is available in a co-pendingapplication, U.S. patent application Ser. No. 12/161,192, published asU.S. Patent Application Publication US 2010/0061984, which is a U.S.National Phase Application of PCT/US2007/001677, “Compositions andMethods for Modulation of Suppressor T Cell Activation,” which isincorporated by references for all purposes herein.

SUMMARY

Importantly, the present invention describes small molecules thatachieve the same goal, namely inhibition of TIP60's active site whilemaintaining its scaffolding ability. As a result, TIP60 binds to FOXP3and recruits p300 but auto-acetylation and subsequent dissociation ofthe complex is markedly decreased, leading to increased overall FOXP3acetylation and Treg suppressive functions.

One embodiment of this invention is a pharmaceutical compositioncomprising an effective amount of a compound having a structure ofFormula 1:

wherein Ar₁ and Ar₂ are each independently C₆-C₁₀ aryl or C₃-C₁₀heteroaryl;R₁ and R₃ are each independently at each occurrence alkyl, halo,hydroxyl, cyano, nitro, or alkoxy;R₂ is H or alkyl; andm and n are independently 0, 1, or 2;and at least one pharmaceutically acceptable carrier, diluent, excipientor auxiliary.

More specific embodiments include those wherein the compositionscomprise compounds having a structure according to B7, B7A, B7B, B7C, orB7G:

Other embodiments include those wherein said compositions inhibitshistone acetyltransferase (HAT) activity or expression. Still otherembodiments include those wherein said compositions that inhibits HATactivity or expression contain a compound that inhibits TIP60 activityor expression.

In certain embodiments, the compositions are separately adapted fororal, parenteral (including intratumor, intravenous, subcutaneous,intraperitoneal, or intramuscular administration), nasal,intrabronchial, or topical administration.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. is a cartoon representation of the mode of interaction betweenthe target inhibitor B7 and TIP60-Acetyl-CoA complex, wherein the B7 isbelieved to inhibit TIP60 non-competitively by forming a functionallyimpaired complex with TIP60 and Acetyl-CoA.

FIG. 2. is a representation of the crystal structure of TIP60-Acetyl-CoAcomplex, where the arrow points to cavity believed where the inhibitorsinteract.

FIG. 3. is graphical representation of FOXP3-TIP60 interaction studiedby means of Surface Plasmon Resonance (SPR) spectroscopy. The N-terminalfragment of FOXP3 was tested for binding to TIP60 in a range ofconcentrations from 2 to 20 uM. The obtained dose response curves areshown in FIG. 3. Curve fitting to a 1:1 binding model was used toestimate the association (k_(on)) and dissociation (k_(off)) rateconstants. The dissociated constant of about 5 uM was then calculated asa k_(off)/k_(on) ratio.

FIG. 4. are Western blot plots showing that p300 promotes TIP60auto-acetylation, and in turn decreases interaction of TIP60 and p300.293T cells were cotransfected with p300 and TIP60 or TIP60 mutant. After24 h, cells were treated with HDACi (400 nM TSA & 10 mM NAD) for 4 h.Cell lysates were analyzed by Western blot using HA-HRP (lowest panel),or immunoprecipitated with anti-FLAG, followed by blotting withacetyl-lysine (first panel), HA-HRP (second panel) or FLAG-HRP (thirdpanel). The plots show particularly the co-precipitation (and socomplexation) of the TIP60mut and p300 when present together.

FIG. 5. show that TIP60 and p300 cooperate to promote FOXP3 acetylation.293T cells were cotransfected with constant amount of FOXP3 & p300, andincreasing amounts of TIP60, then immunoprecipitated with anti-HA,followed by western blot using acetylated-lysine (upper) or HA-HRP(lower).

FIG. 6. show cooperative TIP60/p300 interactions that dynamicallyregulate the HAT activity of p300 and subsequently the extent of FOXP3acetylation. Lysine acetylation is shown by the label “Ac”.

FIG. 7 show a characterization of TIP60dn mouse (indicative of howeffective an ideal TIP60 inhibitor small molecule might be).

FIG. 8. are flow cytometry dataplots showing the enhancement of TIP60inhibitor B7 suppression of Treg in vitro. Individual peaks reflectnumber of cells divisions under the standard test conditions (3 days),where the cell populations are dyed with CFSE (carboxyfluorescendiacetate, succimidyl ester). FIG. 8A are the data for DMSO without B7;FIG. 8B are the data for B7-1000x; FIG. 8C are the data for B7-4000x;and FIG. 8D are the data for B7-8000. In each case, the1000x/4000x/8000x refers to the dilution factor of the B7, from anoriginal 10 mM solution of B7; e.g, 1000x represents a solution dilutedby a factor of 1000, so as to result in a 10 micromolar solution. Thenumbers in the upper left corner of the plot (e.g., 88, 81, 74, 65, and37 of FIG. 8A) refer to the measured percentage of proliferated cells.

FIG. 9. are Western blot dataplots showing B7c intervening with FOXP3bound on chromatin in SZ-4 T cells.

FIG. 10. are flow cytometry dataplots showing the enhancement of TIP60inhibitors B7 suppression of Treg in vitro. FIG. 10A are the data forDMSO without any B7-type inhibitor; FIG. 10B are the data for B7-500x;FIG. 10C are the data for B7A-500x; FIG. 10D are the data for B7B-500;and FIG. 10D are the data for B7C-500x. In each case, the 500x refers tothe dilution factor of the B7/B7A/B7B/B7C, from an original 10 mMsolution of the compound; e.g, 500x represents a solution diluted by afactor of 500, so as to result in a 20 micromolar solution.

FIG. 11 illustrate the effect of B7G on TIP60. TIP60 activity wasassessed by examining acetylation of TIP60 or its substrate FOXP3. BothTIP60 and FOXP3 were overexpressed in 293T cells. Acetylated proteinswere first immunoprecipitated with the acetyl-lysine specific antibodyAc-K and then blotted with HRP conjugated antibodies to expression tags(HA for FOXP3 or FLAG for TIP60).

FIGS. 12A-C are the result of experiments described in Example 5. Inparticular, FIG. 12A shows colon tissue sections of mice taken 3 weeksafter the mice were exposed to 5 days of dextrane sodium sulfiteirritants. FIG. 12B shows colon tissue sections of mice after theadoptive transfer experiments of Example 4. FIG. 12C provide graphicaldata for weight loss, bleeding score, and stool consistency (i.e.,higher score reflects higher levels of diarrhea), wherein the mice werefirst subjected to DSS irritation, then subset populations wereremedially treated after 8 days with the compound having Structure B7.

DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

The present invention may be understood more readily by reference to thefollowing detailed description taken in connection with the accompanyingFigures and Examples, which form a part of this disclosure. It is to beunderstood that this invention is not limited to the specific products,methods, conditions or parameters described and/or shown herein, andthat the terminology used herein is for the purpose of describingparticular embodiments by way of example only and is not intended to belimiting of any claimed invention. Similarly, any description as to apossible mechanism or mode of action or reason for improvement is meantto be illustrative only, and the invention herein is not to beconstrained by the correctness or incorrectness of any such suggestedmechanism or mode of action or reason for improvement. Throughout thistext, it is recognized that the descriptions refer both to the compoundsand to the resulting pharmaceutical compositions and methods ofmanufacture and use.

Importantly, the present invention describes pharmaceutical compositionscontaining at least one small molecule that achieve the same goal,namely inhibition of TIP60's active site while maintaining itsscaffolding ability. As a result, TIP60 binds to FOXP3 and recruits p300but auto-acetylation and subsequent dissociation of the complex ismarkedly decreased, leading to increased overall FOXP3 acetylation andTreg suppressive functions.

The present invention relates to compounds and pharmaceuticalcompositions useful for inhibiting the functions of TIP60 and to methodsof using such compounds and compositions in the treatment of anindividual suffering, for example, from ulcerative colitis and otherirritable bowel diseases.

The invention teaches pharmaceutical compositions comprising compoundshaving the general structure of Formula I:

wherein Ar₁ and Ar₂ are each independently C₆-C₁₀ aryl or C₃-C₁₀heteroaryl;R₁ and R₃ are each independently at each occurrence alkyl, halo,hydroxyl, cyano, nitro, or alkoxy;R₂ is H or alkyl; andm and n are independently 0, 1, 2, or 3.

As used herein, the terms “aryl” refers to a carbocyclic (all carbon)ring that has a fully delocalized pi-electron system. The “aryl” groupcan be made up of two or more fused rings (rings that share two adjacentcarbon atoms). When the aryl is a fused ring system, then the ring thatis connected to the rest of the molecule has a fully delocalizedpi-electron system. The other ring(s) in the fused ring system may ormay not have a fully delocalized pi-electron system. Examples of arylgroups include, but are not limited to, benzene, naphthalene andazulene.

As used herein, “heteroaryl” refers to a ring that contains one or moreheteroatoms selected from the group consisting of nitrogen, oxygen andsulfur in the ring and that has a fully delocalized pi-electron system.The descriptor C₃-C₁₀ or C₅-C₁₀ refers to the number of carbon atoms, inaddition to the necessary heteroatoms, in the system. The “heteroaryl”group can be made up of two or more fused rings (rings that share twoadjacent carbon atoms). When the heteroaryl is a fused ring system, thenthe ring that is connected to the rest of the molecule has a fullydelocalized pi-electron system. The other ring(s) in the fused ringsystem may or may not have a fully delocalized pi-electron system.Examples of heteroaryl rings include, but are not limited to, furan,thiophene, phthalazinone, pyrrole, oxazole, thiazole, imidazole,pyrazole, isoxazole, isothiazole, triazole, thiadiazole, pyran,pyridine, pyridazine, pyrimidine, pyrazine, indole, isoindole,isoquinoline and triazine.

As used herein, “alkyl” refers to a straight, branched chain, or cyclicfully saturated (no double or triple bonds) hydrocarbon (all carbon)group. An alkyl group of this invention may comprise from 1-6 carbonatoms, that is, designated as a “C₁ to C₆ alkyl.” C₁ to C₆ alkyls arepreferred. C₁ to C₃ alkyls are more preferred, and C₁ alkyl is mostpreferred. Examples of alkyl groups include, without limitation, methyl,ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, tert-butyl,amyl, tert-amyl, hexyl, heptyl, octyl, nonyl, decyl, undecyl anddodecyl. Alkyl groups can be partially or fully fluorinated.

As used herein, “halo” and “halogen” refer to the fluoro, chloro, bromoor iodo atoms. Preferred halogens are chloro and fluoro.

Also as used herein, “alkoxy” refers to an “—O—R” group, where Rrepresents alkyl, as defined above. Where two alkoxy groups are adjacentto one another, they may be alicyclic or together form a fused cyclicring system, e.g.,

As used herein, “hydroxyl” refers to an “—OH” group, and those groupswherein the “—OH” group also contains a hydroxyl protecting group. Asused herein, a “hydroxyl protecting group” refers to a readily cleavablegroup that replaces the hydrogen of the hydroxyl group, such as, withoutlimitation, tetrahydropyranyl, 2-methoxypropyl, 1-ethoxyethyl,methoxymethyl, 2-methoxyethoxymethyl, methylthiomethyl, t-butyl, t-amyl,trityl, 4-methoxytrityl, 4,4′-dimethoxytrityl, 4,4′,4″-trimethoxytrityl,benzyl, allyl, trimethylsilyl, (t-butyl)dimethylsilyl, and2,2,2-trichloroethoxycarbonyl. The species of hydroxyl protecting groupsis not critical so long as the derivatized hydroxyl group is stable tothe conditions of subsequent reaction(s) and can be removed at theappropriate point without disrupting the remainder of the molecule.Further examples of hydroxy-protecting groups are described by C. B.Reese and E. Haslam, “Protective Groups in Organic Chemistry,” J. G. W.McOmie, Ed., Plenum Press, New York, N.Y., 1973, Chapters 3 and 4,respectively, and T. W. Greene and P. G. M. Wuts, “Protective Groups inOrganic Synthesis,” 2nd ed., John Wiley and Sons, New York, N.Y., 1991,Chapters 2 and 3, both of which are incorporated by reference herein forthis purpose.

As used herein, “cyano” refers to a “—C≡N” group.

As used herein, “nitro” refers to an “—NO₂” group.

Throughout the present disclosure, when a particular compound comprisesa chiral center, the scope of the present disclosure also includescompositions comprising the racemic mixture of the two enantiomers, aswell as compositions comprising each enantiomer individuallysubstantially free of the other enantiomer. Thus, for example,contemplated herein is a composition comprising the S enantiomersubstantially free of the R enantiomer, or a composition comprising theR enantiomer substantially free of the S enantiomer. By “substantiallyfree” it is meant that the composition comprises less than 10%, or lessthan 8%, or less than 5%, or less than 3%, or less than 1% of the minorenantiomer. If the particular compound comprises more than one chiralcenter, the scope of the present disclosure also includes compositionscomprising a mixture of the various diastereomers, as well ascompositions comprising each diastereomer substantially free of theother diastereomers. The recitation of a compound, without reference toany of its particular diastereomers, includes compositions comprisingall four diastereomers, compositions comprising the racemic mixture ofR,R and S,S isomers, compositions comprising the racemic mixture of R,Sand S,R isomers, compositions comprising the R,R enantiomersubstantially free of the other diastereomers, compositions comprisingthe S,S enantiomer substantially free of the other diastereomers,compositions comprising the R,S enantiomer substantially free of theother diastereomers, and compositions comprising the S,R enantiomersubstantially free of the other diastereomers.

When a tautomer of the compound of the Formula I exists, embodiments ofthe present invention include any possible tautomers andpharmaceutically acceptable salts thereof, and mixtures thereof, exceptwhere specifically drawn or stated otherwise The disclosure and claimsof the present invention are based on the known general principles ofchemical bonding. It is understood that the claims do not encompassstructures known to be unstable or not able to exist based on theliterature.

Additional embodiments include those wherein Ar₁ and Ar₂ are eachindependently C₆-C₁₀ aryl or C₃-C₁₀ heteroaryl.

The invention also includes those compounds having a structure ofFormula II or Formula III:

where m and n are independently 1 or 2.

In other embodiments, the present invention also includes thosecompounds having a structure of Formula IIA, IIIA, IVA, or IVB:

Additional embodiments include those wherein any of the precedingcompounds specifically contain H in the R₂ position and/or where R₃ isindependently halo, generally, and chloro or fluoro, specifically, ateach occurrence.

Still additional embodiments include any or all of the precedingcompounds R₁ is independently C₁₋₃ alkyl, halo, or C₁₋₃ alkoxy, at eachoccurrence.

Additional embodiments include those wherein any of the precedingcompounds specifically contain H in the R₂ position and/or where R₁ isindependently halo, generally, and chloro or fluoro, specifically, ateach occurrence.

Still additional embodiments include any or all of the precedingcompounds R₃ is independently C₁₋₃ alkyl, halo, or C₁₋₃ alkoxy, at eachoccurrence.

Other embodiments include the compounds having the structure of FormulaB7, B7A, B7B, B7C, or B7G:

These compounds can be prepared from conveniently available startingmaterials using conventional synthetic methods (including step-wisenucleophilic substitution reactions) as exemplified by the followinggeneral scheme:

(Note that reference to Compounds of Formula I also comprise all of thesubgenera of species described above). A search of the internet showsthat the raw materials, a number of intermediates, and some of the finalproduct shown in this Scheme are, in fact, commercially available from avariety of commercial sources. The specific compounds B7, B7A, B7B, andB7C, for example, are available from Maybridge Chemical, part of ThermoFisher Scientific, catalog numbers HTS10908 (B7); HTS10905 (B7A);HTS10906 (B7B) and HTS10909 (B7C). The compound identified as B7G isavailable from Enamine Ltd (Catalogue # T5337455).

The present invention also includes those embodiments of the compoundsof Formula I themselves, excepting those specific compounds alreadyknown. That is, a pharmaceutically tolerable analog or homolog of acompound described herein as B7, B7A, B7B, B7C or B7G.

The present invention includes those pharmaceutical compositionscomprising any one of the preceding compounds, as well as anypharmaceutically acceptable salt or solvate which may be derived fromthese compounds and their use in patient treatment. The term“pharmaceutically acceptable salt” means those salts of compounds of theinvention that are safe and effective for use in a subject and thatpossess the desired biological activity. Pharmaceutically acceptablesalts include salts of acidic or basic groups present in compounds ofthe invention. Pharmaceutically acceptable acid addition salts include,but are not limited to, hydrochloride, hydrobromide, hydroiodide,nitrate, sulfate, bisulfate, phosphate, acid phosphate, isonicotinate,acetate, lactate, salicylate, citrate, tartrate, pantothenate,bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate,gluconate, glucaronate, saccharate, formate, benzoate, glutamate,methanesulfonate, ethanesulfonate, benzensulfonate, andp-toluenesulfonate salts. Such salts may be prepared by contacting thebase compound with the corresponding organic or inorganic acid.

Pharmaceutical compositions may be formulated in conventional mannerusing one or more physiologically acceptable carriers, diluents,excipients or auxiliaries which facilitate processing of the activecompound into preparations which can be used pharmaceutically by onehaving ordinary skill in the art with compositions selected dependingupon the chosen mode of administration. Such compositions are preparedin accordance with acceptable pharmaceutical procedures, such asdescribed in Remingtons Pharmaceutical Sciences, 17th edition, ed.Alfonoso R. Gennaro, Mack Publishing Company, Easton, Pa. (1985) astandard reference text in this field, which is incorporated herein byreference. Pharmaceutically acceptable carriers are those that arecompatible with the other ingredients in the formulation andbiologically acceptable.

The pharmaceutical compositions of the present invention may beadministered by any means that enables the active agent to reach theagent's site of action in the body of a mammal. Pharmaceuticalcompositions may be administered orally or parenterally, i.e.,intratumor, intravenous, subcutaneous, intramuscular, etc. The compoundsof this invention may be administered neat or in combination withconventional pharmaceutical carriers, the proportion of which isdetermined by the solubility and chemical nature of the compound, chosenroute of administration and standard pharmacological practice. Thepharmaceutical carrier may be solid or liquid.

The compounds of the invention may be administered orally. Oraladministration may involve swallowing, so that the compound enters thegastrointestinal tract, or buccal or sublingual administration may beemployed by which the compound enters the blood stream directly from themouth.

Formulations suitable for oral administration include solid formulationssuch as tablets, capsules containing particulates, liquids, or powders,lozenges (including liquid-filled), chews, multi- and nano-particulates,gels, solid solution, liposome, films (including muco-adhesive), ovules,sprays and liquid formulations.

Liquid formulations include suspensions, solutions, syrups and elixirs.Such formulations may be used as fillers in soft or hard capsules andtypically include a carrier, for example, water, ethanol, polyethyleneglycol, propylene glycol, methylcellulose, or a suitable oil, and one ormore emulsifying agents and/or suspending agents.

For topical administration the compounds of the invention may beformulated as gels, hydrogels, lotions, solutions, creams, ointments,dusting powders, dressings, foams, films, skin patches, wafers,implants, sponges, fibers, bandages and microemulsions. Liposomes mayalso be used. Typical carriers include alcohol, water, mineral oil,liquid petrolatum, white petrolatum, glycerin, polyethylene glycol andpropylene glycol. Penetration enhancers may be incorporated; see, forexample, J Pharm Sci, 88 (10), 955-958 by Finnin and Morgan (October1999), which is incorporated by reference in its entirety.

Formulations for topical administration may be formulated to beimmediate and/or modified release. Modified release formulations includedelayed-, sustained-, pulsed-, controlled-, targeted and programmedrelease.

Systemic formulations include those designed for administration byinjection, e.g. subcutaneous, intravenous, intramuscular, intrathecal orintraperitoneal injection, as well as those designed for transdermal,transmucosal, oral or pulmonary administration.

For injection, the compounds of the invention may be formulated inaqueous solutions, preferably in physiologically compatible buffers suchas Hanks solution, Ringer's solution, or physiological saline buffer.The solution may contain formulatory agents such as suspending,stabilizing and/or dispersing agents.

Alternatively, the compounds may be in powder form for constitution witha suitable vehicle, e.g., sterile pyrogen-free water, before use

Applicable solid carriers can include one or more substances which mayalso act as flavoring agents, lubricants, solubilizers, suspendingagents, fillers, glidants, compression aids, binders ortablet-disintegrating agents or an encapsulating material. In powders,the carrier is a finely divided solid which is in admixture with thefinely divided active ingredient. In tablets, the active ingredient ismixed with a carrier having the necessary compression properties insuitable proportions and compacted in the shape and size desired. Thepowders and tablets preferably contain up to 99% of the activeingredient. Suitable solid carriers include, for example, calciumphosphate, magnesium stearate, talc, sugars, lactose, dextrin, starch,gelatin, cellulose, methyl cellulose, sodium carboxymethyl cellulose,polyvinylpyrrolidine, low melting waxes and ion exchange resins.

For tablet dosage forms, depending on dose, the drug may make up from 1wt % to 80 wt % of the dosage form, more typically from 5 wt % to 60 wt% of the dosage form. In addition to the drug, tablets generally containa disintegrant. Examples of disintegrants include sodium starchglycolate, sodium carboxymethyl cellulose, calcium carboxymethylcellulose, croscarmellose sodium, crospovidone, polyvinylpyrrolidone,methyl cellulose, microcrystalline cellulose, lower alkyl-substitutedhydroxypropyl cellulose, starch, pregelatinized starch and sodiumalginate. Generally, the disintegrant will comprise from 1 wt % to 25 wt%, preferably from 5 wt % to 20 wt % of the dosage form.

Binders are generally used to impart cohesive qualities to a tabletformulation. Suitable binders include microcrystalline cellulose,gelatin, sugars, polyethylene glycol, natural and synthetic gums,polyvinylpyrrolidone, pregelatinized starch, hydroxypropyl cellulose andhydroxypropyl methylcellulose. Tablets may also contain diluents, suchas lactose (monohydrate, spray-dried monohydrate, anhydrous and thelike), mannitol, xylitol, dextrose, sucrose, sorbitol, microcrystallinecellulose, starch and dibasic calcium phosphate dihydrate.

Tablets may also optionally include surface active agents, such assodium lauryl sulfate and polysorbate 80, and glidants such as silicondioxide and talc. When present, surface active agents are typically inamounts of from 0.2 wt % to 5 wt % of the tablet, and glidants typicallyfrom 0.2 wt % to 1 wt % of the tablet.

Tablets also generally contain lubricants such as magnesium stearate,calcium stearate, zinc stearate, sodium stearyl fumarate, and mixturesof magnesium stearate with sodium lauryl sulphate. Lubricants generallyare present in amounts from 0.25 wt % to 10 wt %, preferably from 0.5 wt% to 3 wt % of the tablet.

Other conventional ingredients include anti-oxidants, colorants,flavoring agents, preservatives and taste-masking agents.

Exemplary tablets contain up to about 80 wt % drug, from about 10 wt %to about 90 wt % binder, from about 0 wt % to about 85 wt % diluent,from about 2 wt % to about 10 wt % disintegrant, and from about 0.25 wt% to about 10 wt % lubricant.

Tablet blends may be compressed directly or by roller to form tablets.Tablet blends or portions of blends may alternatively be wet-, dry-, ormelt-granulated, melt congealed, or extruded before tabletting. Thefinal formulation may include one or more layers and may be coated oruncoated; or encapsulated.

The formulation of tablets is discussed in detail in “PharmaceuticalDosage Forms: Tablets, Vol. 1”, by H. Lieberman and L. Lachman, MarcelDekker, N.Y., N.Y., 1980 (ISBN 0-8247-6918-X), the disclosure of whichis incorporated herein by reference in its entirety.

Solid formulations for oral administration may be formulated to beimmediate and/or modified release. Modified release formulations includedelayed-, sustained-, pulsed-, controlled-, targeted and programmedrelease.

The compounds of the invention may also be administered directly intothe blood stream, into muscle, or into an internal organ. Suitable meansfor parenteral administration include intravenous, intraarterial,intraperitoneal, intrathecal, intraventricular, intraurethral,intrasternal, intracranial, intramuscular and subcutaneous. Suitabledevices for parenteral administration include needle (includingmicroneedle) injectors, needle-free injectors and infusion techniques.

Liquid carriers may be used in preparing solutions, suspensions,emulsions, syrups and elixirs. The active ingredient of this inventioncan be dissolved or suspended in a pharmaceutically acceptable liquidcarrier such as water, an organic solvent, a mixture of both orpharmaceutically acceptable oils or fat. The liquid carrier can containother suitable pharmaceutical additives such as solubilizers,emulsifiers, buffers, preservatives, sweeteners, flavoring agents,suspending agents, thickening agents, colors, viscosity regulators,stabilizers or osmo-regulators. Suitable examples of liquid carriers fororal and parenteral administration include water (particularlycontaining additives as above, e.g. cellulose derivatives, preferablysodium carboxymethyl cellulose solution), alcohols (including monohydricalcohols and polyhydric alcohols e.g. glycols) and their derivatives,and oils (e.g. fractionated coconut oil and arachis oil). For parenteraladministration the carrier can also be an oily ester such as ethyloleate and isopropyl myristate. Sterile liquid carriers are used insterile liquid form compositions for parenteral administration. Theliquid carrier for pressurized compositions can be halogenatedhydrocarbon or other pharmaceutically acceptable propellant.

Liquid pharmaceutical compositions which are sterile solutions orsuspensions can be administered by, for example, intramuscular,intraperitoneal or subcutaneous injection. Sterile solutions can also beadministered intravenously. Oral administration may be either liquid orsolid composition form.

Parenteral formulations are typically aqueous solutions which maycontain excipients such as salts, carbohydrates and buffering agents(preferably to a pH of from 3 to 9), but, for some applications, theymay be more suitably formulated as a sterile non-aqueous solution or asa dried form to be used in conjunction with a suitable vehicle such assterile, pyrogen-free water.

The preparation of parenteral formulations under sterile conditions, forexample, by lyophilization, may readily be accomplished using standardpharmaceutical techniques well known to those skilled in the art.

The solubility of compounds of the invention used in the preparation ofparenteral solutions may be increased by the use of appropriateformulation techniques, such as the incorporation ofsolubility-enhancing agents.

Formulations for parenteral administration may be formulated to beimmediate and/or modified release. Modified release formulations includedelayed-, sustained-, pulsed-, controlled-, targeted and programmedrelease. Thus compounds of the invention may be formulated as a solid,semi-solid, or thixotropic liquid for administration as an implanteddepot providing modified release of the active compound. Examples ofsuch formulations include drug-coated stents and PGLA microspheres.

The compounds of this invention may be administered rectally orvaginally in the form of a conventional suppository, pessary, or enema.Cocoa butter is a traditional suppository base, but various alternativesmay be used as appropriate.

For administration by intranasal or intrabronchial inhalation orinsufflation, the compounds of this invention may be formulated into anaqueous or partially aqueous solution, or as a dry powder (either alone,as a mixture, for example, in a dry blend with lactose, or as a mixedcomponent particle, for example, mixed with phospholipids, such asphosphatidylcholine) from a dry powder inhaler or as an aerosol sprayfrom a pressurized container, pump, spray, atomizer (preferably anatomizer using electrohydrodynamics to produce a fine mist), ornebulizer, with or without the use of a suitable propellant, such as1,1,1,2-tetrafluoroethane or 1,1,1,2,3,3,3-heptafluoropropane. Forintranasal use, the powder may include a bioadhesive agent, for example,chitosan or cyclodextrin.

The pressurized container, pump, spray, atomizer, or nebulizer containsa solution or suspension of the compound(s) of the invention comprising,for example, ethanol, aqueous ethanol, or a suitable alternative agentfor dispersing, solubilizing, or extending release of the active, apropellant(s) as solvent and an optional surfactant, such as sorbitantrioleate, oleic acid, or an oligolactic acid.

Prior to use in a dry powder or suspension formulation, the drug productis micronized to a size suitable for delivery by inhalation (typicallyless than 5 microns). This may be achieved by any appropriatecomminuting method, such as spiral jet milling, fluid bed jet milling,supercritical fluid processing to form nanoparticles, high pressurehomogenisation, or spray drying.

Capsules (made, for example, from gelatin or HPMC), blisters andcartridges for use in an inhaler or insufflator may be formulated tocontain a powder mix of the compound of the invention, a suitable powderbase such as lactose or starch and a performance modifier such asl-leucine, mannitol, or magnesium stearate. The lactose may be anhydrousor in the form of the monohydrate, preferably the latter. Other suitableexcipients include dextran, glucose, maltose, sorbitol, xylitol,fructose, sucrose and trehalose.

A suitable solution formulation for use in an atomizer usingelectrohydrodynamics to produce a fine mist may contain from 1 μg to 20mg of the compound of the invention per actuation and the actuationvolume may vary from 1 .mu.L to 100 μL. A typical formulation includes acompound of the invention, propylene glycol, sterile water, ethanol andsodium chloride. Alternative solvents which may be used instead ofpropylene glycol include glycerol and polyethylene glycol.

Suitable flavors, such as menthol and levomenthol, or sweeteners, suchas saccharin or saccharin sodium, may be added to those formulations ofthe invention intended for inhaled/intranasal administration.

Formulations for inhaled/intranasal administration may be formulated tobe immediate and/or modified release using, for example,poly(DL-lactic-coglycolic acid (PGLA). Modified release formulationsinclude delayed-, sustained-, pulsed-, controlled-, targeted andprogrammed release.

The compounds of this invention may also be administered transdermallythrough the use of a transdermal patch containing the active compoundand a carrier that is inert to the active compound, is non-toxic to theskin, and allows delivery of the agent for systemic absorption into theblood stream via the skin. The carrier may take any number of forms suchas creams and ointments, pastes, gels, and occlusive devices. The creamsand ointments may be viscous liquid or semisolid emulsions of either theoil-in-water or water-in-oil type. Pastes comprised of absorptivepowders dispersed in petroleum or hydrophilic petroleum containing theactive ingredient may also be suitable. A variety of occlusive devicesmay be used to release the active ingredient into the blood stream suchas a semipermeable membrane covering a reservoir containing the activeingredient with or without a carrier, or a matrix containing the activeingredient. Other occlusive devices are known in the literature.

Preferably the pharmaceutical composition is in unit dosage form, e.g.as tablets, capsules, powders, solutions, suspensions, emulsions,granules, or suppositories. In such form, the composition is sub-dividedin unit dose containing appropriate quantities of the active ingredient;the unit dosage forms can be packaged compositions, for example packetedpowders, vials, ampoules, prefilled syringes or sachets containingliquids. The unit dosage form can be, for example, a capsule or tabletitself, or it can be the appropriate number of any such compositions inpackage form.

Pharmaceutical compositions suitable for use in the methods disclosedherein include compositions where the active ingredients are containedin an amount effective to achieve its intended purpose. Morespecifically, a therapeutically effective amount means an amount ofcompound effective to prevent, alleviate or ameliorate symptoms ofdisease or prolong the survival of the subject being treated.Determination of a therapeutically effective amount is well within thecapability of those skilled in the art.

Dosage varies depending upon known factors such as the pharmacodynamiccharacteristics of the particular agent, and its mode and route ofadministration; age, health, and weight of the recipient; nature andextent of symptoms, kind of concurrent treatment, frequency oftreatment, and the effect desired. The dosage requirements vary with theparticular compositions employed, the route of administration, theseverity of the symptoms presented and the particular subject beingtreated. Based on the results obtained in the standard pharmacologicaltest procedures, projected daily dosages of active compound would be0.02 μg/kg-750 μg/kg. Treatment will generally be initiated with smalldosages less than the optimum dose of the compound. Thereafter thedosage is increased until the optimum effect under the circumstances isreached; precise dosages for oral, parenteral, nasal, or intrabronchialadministration will be determined by the administering physician basedon experience with the individual subject treated.

Certain aspects of the present invention teach methods of treating apatient in need thereof comprising administering a pharmaceuticallyeffective amount of at least one compound of Formula I. As used withinthe following discussion, the term “at least one compound of Formula I”is intended to include all the specific embodiments described previouslyin the specification as relating to the genus and species of thecompound of Formula I, as well as corresponding pharmaceuticalcompositions, salts and solvates.

The term “administering” in the context of administering a compoundrefers to any or all of the steps including preparing a formulation, asdiscussed herein, containing the compound being administered;prescribing or indicating the need to take the compound beingadministered; providing a composition containing the compound beingadministered; and/or ingesting by or applying to a subject or a cell ororgan within the subject, by any known method. For example, a solutioncontaining the compound can be injected to the subject or be added tothe medium containing the cells, or the subject can orally ingest aformulation containing the compound. The term “contacting” refers tobringing the subject or the cell into contact with the compound. Thus, aformulation of a prodrug can be administered to a subject, whereupon theprodrug undergoes metabolism. The metabolite is then either in thesystemic circulation or within the cytoplasm. In this situation, theprodrug is “administered” to the subject, but both the subject and thecells are “contacted” with the metabolite.

The term “treating” or “treatment” does not necessarily mean total cure.Any alleviation of any undesired signs or symptoms of the disease to anyextent or the slowing down of the progress of the disease can beconsidered treatment. Furthermore, treatment may include acts that mayworsen the patient's overall feeling of well-being or appearance.Treatment may also include lengthening the life of the patient, even ifthe symptoms are not alleviated, the disease conditions are notameliorated, or the patient's overall feeling of well-being is notimproved.

Similarly, the term “preventing” does not require total prevention of adisease or condition, but also includes the inhibition or regression ofprogress of the disease or condition said to be prevented.

In certain aspects, disclosed herein are methods of treating a disorderin a subject comprising administering to the subject a therapeuticallyeffective amount of at least one compound of Formula I. Said embodimentsmay also include identifying the subject in need of such treatment,though the invention is intended to encompass the effect of theadministration and not necessarily the conscious intent of theadministrator. The disorders include autoimmune disorders that is causedby a lack, deficiency, or underperformance of functional suppressor Tcells. Specific examples of disorders of the present invention includemultiple sclerosis, diabetes mellitus, rheumatoid arthritis, lupus,Crohn's disease, Hashimoto's disease, polymyositis, inflammatory boweldisease, colitis, scleroderma, oophoritis, thyroiditis, Grave's disease,dermatomyositis, pemphigus vulgaris, myasthenia gravis, hemolyticanemia, or Sjogren's disease, and especially Crohn's disease,inflammatory bowel disease, or colitis.

Data from NIH, the American Autoimmune Related Diseases Association andelsewhere indicate that ˜24 million Americans live with one or moretypes of the currently 80-100 recognized autoimmune diseases (10-12).Autoimmunity costs the nation $86 billion/year. Moreover, ˜75% of casesof autoimmune diseases occur in women, and autoimmune diseases are the4^(th) largest cause of disability among US women. Broken down further,autoimmune thyroiditis affects 40/1000 American women, psoriasisaffects >20/1000 Americans, rheumatoid arthritis affects about 10/1000Americans, SLE affects 4/1000 young African-American women, and IBD,multiple sclerosis and type I diabetes each affect ˜1.5/1000 Americans.On a smaller scale, there are also important unmet needs in terms ofregulation of immunity in transplant recipients, with 27,578 patientsreceiving organ transplants in 2007, and 173,339 patients currentlyliving with a functional organ transplant (UNOS data). Even with complexcurrent immunosuppressive regimens, 5-year graft survival rates are 68%for cadaveric donor kidneys and 73% for heart grafts, and there are highrates of toxicity associated with these protocols. These areas ofautoimmunity and transplantation are further interrelated in thatautoimmune diseases are a major cause of endstage organ failurerequiring transplantation (e.g. kidney, liver), and some forms ofendstage autoimmunity would be treated by tissue transplantation if lesstoxic immunosuppressive regimens were available so as to controltransplant rejection without having a major negative impact on the host(e.g. islet grafts for type 1 diabetes). It is envisioned that thetreatments described in the present invention can be used to treat theseailments or conditions.

In certain embodiments, the at least one compound of Formula I isadministered remedially, after the development of the disease. In otherembodiments, the at least one compound of Formula I is administeredprophylatically, before the development of the disease, for example, insubjects otherwise at risk of developing the disease or condition,whether that risk is caused by genetics, injury, or other circumstance.

The exact formulation, route of administration and dosage for thepharmaceutical compositions disclosed herein can be chosen by theindividual physician in view of the patient's condition. (See e.g.,Fingl et al. 1975, in “The Pharmacological Basis of Therapeutics”, Ch. 1p. 1). Typically, the dose about the composition administered to thepatient can be from about 0.5 to 1000 mg/kg of the patient's bodyweight, or 1 to 500 mg/kg, or 10 to 500 mg/kg, or 50 to 100 mg/kg of thepatient's body weight. The dosage may be a single one or a series of twoor more given in the course of one or more days, as is needed by thepatient. Note that for almost all of the specific compounds mentioned inthe present disclosure, human dosages for treatment of at least somecondition have been established. Thus, in most instances, the methodsdisclosed herein will use those same dosages, or dosages that arebetween about 0.1% and 500%, or between about 25% and 250%, or between50% and 100% of the established human dosage. Where no human dosage isestablished, as will be the case for newly discovered pharmaceuticalcompounds, a suitable human dosage can be inferred from ED₅₀ or ID₅₀values, or other appropriate values derived from in vitro or in vivostudies, as qualified by toxicity studies and efficacy studies inanimals. For systemic administration, a therapeutically effective dosecan be estimated initially from in vitro assays.

Although the exact dosage will be determined on a drug-by-drug basis, inmost cases, some generalizations regarding the dosage can be made. Thedaily dosage regimen for an adult human patient may be, for example, anoral dose of between 0.1 mg and 500 mg of each ingredient, preferablybetween 1 mg and 250 mg, e.g. 5 to 200 mg or an intravenous,subcutaneous, or intramuscular dose of each ingredient between 0.01 mgand 100 mg, preferably between 0.1 mg and 60 mg, e.g. 1 to 40 mg of eachingredient of the pharmaceutical compositions disclosed herein or apharmaceutically acceptable salt thereof calculated as the free base,the composition being administered 1 to 4 times per day. Alternativelythe compositions disclosed herein may be administered by continuousintravenous infusion, preferably at a dose of each ingredient up to 400mg per day. Thus, the total daily dosage by oral administration of eachingredient will typically be in the range 1 to 2000 mg and the totaldaily dosage by parenteral administration will typically be in the range0.1 to 400 mg. Suitably the compounds will be administered for a periodof continuous therapy, for example for a week or more, or for months oryears.

Dosage amount and interval may be adjusted individually to provideplasma levels of the active moiety, which are sufficient to maintain themodulating effects, or minimal effective concentration (MEC). The MECwill vary for each compound but can be estimated from in vitro data.Dosages necessary to achieve the MEC will depend on individualcharacteristics and route of administration. However, HPLC assays orbioassays can be used to determine plasma concentrations.

Dosage intervals can also be determined using MEC value. Compositionsshould be administered using a regimen, which maintains plasma levelsabove the MEC for 10-90% of the time, preferably between 30-90% and mostpreferably between 50-90%.

In cases of local administration or selective uptake, the effectivelocal concentration of the drug may not be related to plasmaconcentration. One having skill in the art will be able to optimizetherapeutically effective local dosages without undue experimentation.

The amount of composition administered will, of course, be dependent onthe subject being treated, on the subject's weight, the severity of theaffliction, the manner of administration and the judgment of theprescribing physician.

The compositions may, if desired, be presented in a pack or dispenserdevice, which may contain one or more unit dosage forms containing theactive ingredient. The pack may for example comprise metal or plasticfoil, such as a blister pack. The pack or dispenser device may beaccompanied by instructions for administration. The pack or dispensermay also be accompanied with a notice associated with the container inform prescribed by a governmental agency regulating the manufacture,use, or sale of pharmaceuticals, which notice is reflective of approvalby the agency of the form of the drug for human or veterinaryadministration. Such notice, for example, may be the labeling approvedby the U.S. Food and Drug Administration for prescription drugs, or theapproved product insert. Compositions comprising a compound disclosedherein formulated in a compatible pharmaceutical carrier may also beprepared, placed in an appropriate container, and labeled for treatmentof an indicated condition.

A co-pending application, U.S. patent application Ser. No. 12/161,192,published as U.S. Patent Application Publication US 2010/0061984, whichis incorporated by reference herein, describes other compositions andmethods for modulation of suppressor T cell activation. It is envisionedthat the present compositions and treatment options may complement thosedescribed in this application, and may also be used as describedtherein.

EXAMPLES Example 1 TIP60/p300/FOXP3 Interactions

The data in FIG. 4 through FIG. 6 show that both TIP60 of the MYSTfamily and p300 of the p300/CBP family positively regulate theacetylation of FOXP3. These proteins, upon activation by discreteextrinsic signals, act to regulate the acetylation levels of FOXP3.Acetylation is also required for the HAT activity of TIP60 and p300, aprocess that is achieved either by auto-acetylation or as a result ofacetylation by other HATs. Both TIP60 and p300 are more active in theiracetylated forms, and p300 promotes the acetylation level of TIP60.Using an enzymatic deficient form of TIP60, this TIP60 mutant was foundnot to be acetylated even in the presence of p300, indicating that p300actually promotes TIP60 auto-acetylation. Interestingly, the interactionof TIP60 and p300 is then decreased when TIP60 is acetylated (FIG. 4).While TIP60 and p300 were both shown to acetylate FOXP3, TIP60 and p300cooperate with each other to promote their acetylation level and thusfacilitating FOXP3 acetylation. Indeed when FOXP3 is present in lowlevel, neither p300 nor TIP60 alone is sufficient to lead to high levelsof FOXP3 acetylation. However, when TIP60 and p300 are combined, strongFOXP3 acetylation was observed (FIG. 5), indicating synergy of TIP60 andp300 in promoting FOXP3 acetylation. These events are modeled in FIG. 6.

Example 2 TIP60 Inhibition by Genetic Means

As a prelude to seeking pharmacologic inhibitors of TIP60 (TIP60i),TIP60 dominant-negative (TIP60dn) mice were developed in which keyresidues in the active site of TIP60 were mutated so as to removecatalytic activity. TIP60dn mice are healthy, breed normally, are notprone to infections or tumors, but have no TIP60 catalytic activity whenassessed using in vitro assays (data not shown). As shown in FIG. 7, Tcells from these mice proliferate normally upon CD3/CD28 mAb-inducedactivation in the absence of Tregs (top panel). These mice also havenormal numbers of FOXP3+CD4+ Tregs (middle panel). However, Tregsisolated form these mice have markedly enhanced Treg function, as shown(lower panel) using standard Treg suppression assays. TIP60dn miceappear normal but their Tregs have enhanced suppressive activity invitro (and in vivo, as shown below).

Example 3 Screening of Pharmacologic TIP60 Inhibitors

Several exemplary compounds of the present invention were screened at 10μg/ml in vitro, at varying Treg:T cell ratios, using standard cellproliferation techniques (3 days, using CSFE dyes). Such techniques arewell understood by those skilled in the art. For example, see Akimova T,Ge G, Golovina T, Mikheeva T, Wang L, Riley J L, Hancock W W.,“Histone/protein deacetylase inhibitors increase suppressive functionsof human FOXP3+ Tregs,” Clin Immunol. 2010 September; 136(3):348-63; TaoR, Hancock W W., Resistance of FOXP3+ regulatory T cells toNur77-induced apoptosis promotes allograft survival,” PLoS One. 2008 May28; 3(5):e2321; Tao R, Wang L, Murphy K M, Fraser C C, Hancock W W.,“Regulatory T cell expression of herpesvirus entry mediator suppressesthe function of B and T lymphocyte attenuator-positive effector Tcells.,” J. Immunol. 2008 May 15; 180(10):6649-55; and Tao R, de ZoetenE F, Ozkaynak E, Chen C, Wang L, Porrett P M, Li B, Turka L A, Olson EN, Greene M I, Wells A D, Hancock W W., “Deacetylase inhibition promotesthe generation and function of regulatory T cells,” Nat. Med. 2007November; 13(11):1299-307 for the protocols used herein. Each of thesereferences is incorporated by reference herein, at least for thispurpose.

T cell proliferation was unimpaired in the absence of Treg cells (seeleft panels in FIG. 8A-D), but was increasingly impaired at increasingTreg concentration (for example, in FIG. 8A, increasing Treg:Tcell ratiofrom 0:1 to 1:1 reduced proliferation from 88% to 37% under otherwiseidentical conditions). The addition of B7 enhanced the Treg activity(decreasing Tcell proliferation) in a dose dependent manner (forexample, compare the effect of increasing B7 dosages in FIGS. 8D-B, from2.5 to 10 μM, on cell proliferation at Treg:Teff=1:1, decreasing from37% to 30% to 25%). Similar effects were seen with the other compoundsstudied (e.g., FIG. 10A-10E). Efficacy was also seen at 1 μg/ml for somecompounds.

Example 4 Inhibition of TIP60 Activity by B7G

TIP60 is known to have acetyl transferase activity to acetylate itself(auto-acetylation) or substrates including FOXP3. To examine activity ofB7G on TIP60, 293T cells were transfected with Flag-tagged TIP60 andHA-tagged FOXP3. 24 hours after transfection, cells were treated withvarious concentrations of B7G for 4 hours before cell lysates werecollected. Acetylated proteins were precipitated by anti-acetyl lysineantibody Ac-K. FOXP3 and TIP60 in the lysate or precipitated by Ac-Kantibody were identified by anti-HA-HRP and anti-FLAG-HRP respectively.As shown in FIG. 11, analysis of total cell lysates indicated that FOXP3and TIP60 expression levels were not significantly affected by B7G (see“Total lysate”, HA-HRP and FLAG-HRP panels). Acetylated FOXP3 andAcetylated TIP60, as indicated by the HA-HRP and FLAG-HRP blots fromAc-K precipitated proteins, were reduced significantly when cells weretreated with 32 μg/ml B7G. At lower concentration, B7G appeared toslightly increase TIP60 auto-acetylation.

Example 5 TIP60 Targeting In Vivo Using Murine IBD Models (FIG. 12)

The TIP60dn mice were used to assess the performance of several TIP60inhibitors, and data were generated in 2 different IBD models with thesemice.

In a first test, the effects of exposure to dextran sodium sulfate (DSS)were evaluated, which results in a partially CD4-dependent inflammatorymodel of colitis, in wildtype (WT) versus TIP60dn C57BL/6 mice (8mice/group). TIP60dn mice showed a marked resistance to development ofcolitis, with decreased histologic evidence of colitis (p=0.005), asseen in FIG. 12A. Also reflective of this inflammation, preliminary datashowed significantly reduced colon lengths for the DMSO control animals(mean lengths=52.7 mm) vs. the TIP60dn mice (mean lengths=64.3 mm) after21 days of treatment (p=0.0019).

Additionally, in a rigorously T cell-dependent model of colitis,purified CD45RBhi Tcells were adoptive transferred into immunodeficientmice. Co-administration of TIP60dn Tregs markedly attenuated thedevelopment of colitis compared to use of WT Tregs, as shownhistologically (p=0.008) in FIG. 12B. Also reflective of thisinflammation, preliminary data showed significantly reduced colonlengths for the animals subjected to co-administered Tcells plus WTTregs (mean lengths=52.7 mm) vs. the mice co-administered with Tcellsplus TIP60dn Tregs (mean lengths=64.3 mm) (p=0.0012). The fully Tcell-dependent adoptive transfer model of colitis lasted 75-100 days,since the onset of disease post-transfer varies.

Data were also generated using the compound of Structure B7 in the DSSmodel, in an experiment designed to test the ability of TIP60 inhibitortherapy to reverse established colitis by waiting until mice had ˜15%weight loss. In marked contrast to use of carrier DMSO alone, TIP60inhibitor therapy (2 mg/kg/d, i.p.) reversed weight loss (p<0.01), bloodin the stool (p<0.01) and diarrhea (p<0.005) (see FIG. 12C) andmaintained colon length (p<0.01). The colon of a single animal treatedwith 3% DSS, then B7 according to this regimen, had a length about 94%of that of an unstressed control animal, whereas the length of the colonof an animal stressed by the 3% DSS but not remediated by B7 was about85% of control. Likewise, the spleen of the remediate animal wascomparable in size to that of the control, whereas the spleen of theunremediated animal was about 3-4 times as large as that of the controlanimal.

As a result of these experiments, the skilled artisan can conclude thatTIP60 targeting can prevent development of colitis (Colitis 1 panel) andprotective effects can be linked solely to the effects on Tregexpression of reduced TIP60 activity (Colitis 2 panel). In addition,markedly beneficial effects are seen using the TIP60 inhibitors oncedisease has been induced (Colitis 3 panel); i.e. the molecules of thepresent invention can be used to treat colitis.

As those skilled in the art will appreciate, numerous modifications andvariations of the present invention are possible in light of theseteachings, and all such are contemplated hereby. For example, inaddition to the embodiments described herein, the present inventioncontemplates and claims those inventions resulting from the combinationof features of the invention cited herein and those of the cited priorart references which complement the features of the present invention.Similarly, it will be appreciated that any described material, feature,or method may be used in combination with any other material, feature,or method.

The disclosures of each patent, patent application, and publicationcited or described in this document are hereby incorporated herein byreference, in their entirety, for all purposes.

1. A pharmaceutical composition comprising a compound having a structureof Formula 1:

wherein Ar₁ and Ar₂ are each independently C₆-C₁₀ aryl or C₃-C₁₀heteroaryl; R₁ and R₃ are each independently at each occurrence alkyl,halo, hydroxyl, cyano, nitro, or alkoxy; R₂ is H or alkyl; and m and nare independently 0, 1, or 2; and at least one pharmaceuticallyacceptable carrier, diluent, excipient or auxiliary.
 2. Thepharmaceutical composition of claim 1, wherein compound inhibits HATactivity or expression.
 3. The pharmaceutical composition of claim 2,wherein the compound that inhibits HAT activity or expression is acompound that inhibits TIP60 activity or expression.
 4. Thepharmaceutical composition of claim 1, wherein Ar₁ and Ar₂ are eachindependently C₆-C₁₀ aryl or C₅-C₁₀ heteroaryl.
 5. The pharmaceuticalcomposition of claim 1, wherein the compound has a structure of FormulaII or Formula III:

where m and n are independently 1 or
 2. 6. The pharmaceuticalcomposition of claim 1, wherein the compound has a structure of FormulaIIA, IIIA, IVA, or IVB:


7. The pharmaceutical composition of claim 1 wherein R₂ is H.
 8. Thepharmaceutical composition of claim 1, wherein R₃ is independently haloat each occurrence.
 9. The pharmaceutical composition of claim 8,wherein R₃ is independently at each occurrence chloro or fluoro.
 10. Thepharmaceutical composition of claim 8, wherein R₁ is independently ateach occurrence C₁₋₃ alkyl, halo, or C₁₋₃ alkoxy.
 11. The pharmaceuticalcomposition of claim 1, wherein R₁ is independently at each occurrencehalo.
 12. The pharmaceutical composition of claim 11, wherein R₁ isindependently at each occurrence chloro or fluoro.
 13. Thepharmaceutical composition of claim 11, wherein R₃ is independently ateach occurrence C₁₋₃ alkyl, halo, or C₁₋₃ alkoxy.
 14. The pharmaceuticalcomposition of claim 1, wherein the compound has a structure of FormulaB7, B7A, B7B, B7C, or B7G:


15. The pharmaceutical composition of claim 1, wherein the compositionis adapted for oral or parenteral administration.
 16. The pharmaceuticalcomposition of claim 15, wherein the composition is in unit dosage form.17. The pharmaceutical composition of claim 15, wherein the compositioncomprises the compound of formula (I) in a therapeutically effectiveamount.
 18. The pharmaceutical composition of claim 16, wherein thecomposition is in the form of a tablet, capsule, powder, solution,suspension, emulsion, granule, or suppository.
 19. The composition ofclaim 1 wherein the pharmaceutically acceptable carrier is a solid. 20.The pharmaceutical composition of claim 1, wherein the formulationadapted for intratumor, intravenous, subcutaneous, intraperitoneal, orintramuscular administration.
 21. (canceled)